Estimation of Age Through Visual Analysis of The Anatomical Surfaces of the Pubic Symphyses and Medial Epiphyses of the Clavicle

Authors

  • Thais Helena dos Santos Salgado Departamento de Saúde Coletiva, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP), SP, Brazil
  • Ana Flavia de Carvalho Cardozo Departamento de Saúde Coletiva, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP), SP, Brazil
  • Alexandre Raphael Deitos Instituto Nacional de Criminalística, Diretoria Técnico-Científica da Polícia Federal, DF, Brazil
  • João Sarmento Pereira Nato Departamento de Saúde Coletiva, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP), SP, Brazil
  • Vanessa Galeggo Arias Pecorari Departamento de Saúde Coletiva, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP), SP, Brazil
  • Luiz Francesquini Júnior Departamento de Saúde Coletiva, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas (UNICAMP), SP, Brazil

DOI:

https://doi.org/10.17063/bjfs12(4)y2025327-339

Keywords:

Forensic anthropology, Skeleton age determination, Bone banks

Abstract

Forensic Anthropology is a crucial auxiliary tool for age estimation in cases involving skeletons with advanced decomposition, mutilation, skeletonization, or fragmentation. Objective: To assess the applicability of qualitative methods for age estimation in a modern Brazilian sample. Morphological analyses of the pubic symphyses and medial epiphyses of the clavicle were conducted using photographic captures (Canon® digital camera and ABFO scale no. 2). The collected data included information such as sex, age, ancestry, and cause of death. Sample selection criteria considered the integrity of the skeletons, preservation quality, and age range between 17 and 65 years. Statistical analysis included chi-square tests to evaluate the association between morphological phases and age ranges, as well as confidence intervals, sensitivity, and specificity. The sample consisted of 15 female skeletons (22 to 65 years old) and 40 male skeletons (17 to 65 years old), with an average and median age of 37 years. Statistical tests showed significance only in the male sample regarding pubic symphysis analysis. The results were promising, although only the male sample in the pubic symphysis analysis showed statistical significance. This study highlights the importance of regional validations and the application of specific methodologies for diverse population samples.

References

Brooks S, Suchey JM. Skeletal age determination based on the os pubis: a comparison of the Acsádi-Nemeskéri and Suchey-Brooks methods. Hum Evol. 1990;5(3):227-38. http://dx.doi.org/10.1007/BF02437238

Milenkovic P, Djukic K, Djonic D, Milovanovic P, Djuric M. Skeletal age estimation based on medial clavicle—a test of the method reliability. Int J Legal Med. 2013;127:667–76. http://dx.doi.org/10.1007/s00414-012-0791-6

Daruge E, Daruge Júnior E, Francesquini Júnior L. Tratado de Odontologia Legal e Deontologia. 2ª ed. São Paulo: Editora Santos; 2019.

Khandare SV, Bhise SS, Shinde AB. Age estimation from cranial sutures – a postmortem study. Int J Healthc Biomed Res. 2015;3:192-202.

Interpol. Disaster Victim Identification Guide. 2018.

Chantharawetchakun T, Vachirawongsakorn V. Age estimation in the Thai male population using epiphyseal union of the medial clavicle. Chiang Mai Med J. 2021;60(2):149-56. http://dx.doi.org/10.12982/CMUMEDJ.2021.13

Villa C, Lynnerup N. Age estimation of skeletal remains: principal methods. Res Rep Forensic Med Sci. 2014;4:3–9. http://dx.doi.org/10.2147/RRFMS.S35660

Vanrell JP. Odontologia Legal e Antropologia Forense. 3ª ed. Rio de Janeiro: Guanabara Koogan; 2019.

Machado CEP, Deitos AR, Velho JÁ, Cunha E. Tratado de Antropologia Forense - Fundamentos e Metodologias Aplicadas à Prática Pericial. Campinas: Millennium; 2022.

Savall F, et al. Age estimation at death using pubic bone analysis of a virtual. Int J Legal Med. 2018;132:609–15. http://dx.doi.org/10.1007/s00414-017-1656-9

Todd T. Age changes in the pubic bone. I. The male white pubis. Am J Phys Anthropol. 1920;3(3):285-334. http://dx.doi.org/10.1002/ajpa.1330030301

Brooks ST. Skeletal age at death: the reliability of cranial and pubic age indicators. Am J Phys Anthropol. 1955;13:567-97. http://dx.doi.org/10.1002/ajpa.1330130403

McKern TW, Stewart TD. Skeletal age changes in young American males analyzed from the standpoint of age identification. Quartermaster Research and Development, Massachusetts; 1957.

Nemeskéri J, Harsányi L, Acsädi G. Methoden zur Diagnose des Lebensalters von Skelettfunden. Anthropol Anz. 1960;1:70-95.

Pattamapaspong N, Madla C, Mekjaidee K, Namwongprom S. Age estimation of a Thai population based on maturation of the medial clavicular epiphysis using computed tomography. Forensic Sci Int. 2015;246:123.e1-5. http://dx.doi.org/10.1016/j.forsciint.2014.10.044

Falys CG, Prangle D. Estimating age of mature adults from the degeneration of the sternal end of the clavicle. Am J Phys Anthropol. 2015;156(2):203-14. http://dx.doi.org/10.1002/ajpa.22639

Houpert T, Rerolle C, Savall F, Telmon N, Saint-Martin P. Is a CT-scan of the medial clavicle epiphysis a good exam to attest to the 18-year threshold in forensic age estimation? Forensic Sci Int. 2016;260:103.e1-3. http://dx.doi.org/10.1016/j.forsciint.2015.12.007

Cerezo-Roma JI, Espinoza POH. Estimating age at death using the sternal end of the fourth ribs from Mexican males. Forensic Sci Int. 2014;236:196.e1-6. http://dx.doi.org/10.1016/j.forsciint.2013.12.044

Landis JR, Koch GG. The measurement of observer agreement for categorical data. Int Biometric Soc. 1977;33(1):159-74. https://doi.org/10.2307/2529310

Langley NR. The lateral clavicular epiphysis: fusion timing and age estimation. Int J Legal Med. 2016;130:511–7. http://dx.doi.org/10.1007/s00414-015-1236-9

Schaefer MC, Black SM. Epiphyseal union sequencing: aiding in the recognition and sorting of commingled remains. J Forensic Sci. 2007;52(2):277-85. https://doi.org/10.1111/j.1556-4029.2006.00381.x

Langley-Shirley N, Jantz RL. A Bayesian approach to age estimation in modern Americans from the clavicle. J Forensic Sci. 2010;55(3):571-83. http://dx.doi.org/10.1111/j.1556-4029.2010.01089.x

Sakaue K. Application of the Suchey–Brooks system of pubic age estimation to recent Japanese skeletal material. Anthropol Sci. 2006;114:59–64. http://dx.doi.org/10.1537/ase.00098

Berg GE. Pubic bone age estimation in adult women. J Forensic Sci. 2008;53(3):569-77. http://dx.doi.org/10.1111/j.1556-4029.2008.00712.x

Sarajlić N, Gradaščević A. Morphological characteristics of pubic symphysis for age estimation of exhumed persons. Bosn J Basic Med Sci. 2012;12(1):51-4. http://dx.doi.org/10.17305/bjbms.2012.2534

Taruffo M. Le prove scientifiche nella recente esperienza statuniense. RFDPC. 1996;8(2):205-50.

Cunha TT, et al. The Brazilian identified human osteological collectionns. Forensic Sci Int. 2018;289:4491-6. https://doi.org/10.1016/j.forsciint.2018.05.040

Published

2025-02-28

How to Cite

dos Santos Salgado, T. H., de Carvalho Cardozo, A. F., Deitos, A. R., Sarmento Pereira Nato, J., Galeggo Arias Pecorari, V., & Francesquini Júnior, L. (2025). Estimation of Age Through Visual Analysis of The Anatomical Surfaces of the Pubic Symphyses and Medial Epiphyses of the Clavicle. Brazilian Journal of Forensic Sciences, Medical Law and Bioethics, 12(4), 327–339. https://doi.org/10.17063/bjfs12(4)y2025327-339

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Section

Artigo Original