Stanley and Acetolysis: Enhanced Method for Pollen Identification and Geolocation of Cocaine Samples
DOI:
https://doi.org/10.17063/bjfs12(2)y2024207-215Keywords:
Forensic palynology, Cocaine, Geolocation, Brazil, Drug traffickingAbstract
The concept of palynological assemblage, a set of all identifiable pollen grains and spores, is very useful in geolocation determinations in Forensic Studies. To identify plant genera, palynologists must recognize detailed aspects of apertures, ornamentation and layers on the cell wall. Pollen and spores recovered from higher purity cocaine samples represent an alternative to indicate drug source regions. After following the method established by Stanley in 1992, we added the acetolysis procedure in samples seized by the Civil Police of the State of Sao Paulo, Brazil, in 2017, in order to enhance morphological traits observation and taxonomical identification of different pollen taxa, allowing reliable environmental and geolocation reconstructions.
References
UNODC (United Nations Office on Drugs and Crime). World Drug Report. Vienna, Austria: United Nations; 2020. 59 p.
Mallette JR, Casale JF. Rapid determination of the isomeric truxillines in illicit cocaine via capillary gas chromatography/flame ionization detection and their use and implication in the determination of cocaine origin and trafficking routes. J Chromatogr A. 2014; 1364:234-40. https://doi.org/10.1016/j.chroma.2014.08.072
Mallette JR, Casale JF, Jordan J, Morello DR, Beyer PM. Geographically sourcing cocaine’s origin – delineation of the nineteen major coca growing regions in South America. Sci Rep. 2016; 6: 23520. https://doi.org/10.1038/srep23520
Nielsen LS, Lindholst C, Villesen P. Cocaine classification using alkaloid and residual solvent profiling. Forensic Sci Int. 2016; 269:42-9. https://doi.org/10.1016/j.forsciint.2016.11.007
Zacca JJ, Botelho ÉD, Vieira ML, Almeida FLA, Ferreira LS, Maldaner AO. Brazilian Federal Police drug chemical profiling: The PeQui project. Sci Justice. 2014; 54:300-6. https://doi.org/10.1016/j.scijus.2014.02.008
Botelho ÉD, Cunha RB, Campos AFC, Maldaner AO. Chemical profiling of cocaine seized by Brazilian Federal Police in 2009-2012: major components. J. Braz. Chem. Soc. 2014; 25(4):611-795. https://doi.org/10.5935/0103-5053.20140008
Erdtman G. Pollen Morphology and Plant Taxonomy - Angiosperms. Stockholm, Sweden: Almqvist and Wiksell; 1952. 539 p. https://doi.org/10.1080/11035895209453507
Bryant VM, Jones JG, Mildenhall DC. Forensic Palynology in The United States of America. Palynology. 1990; 14: 193-208. https://doi.org/10.1080/01916122.1990.9989380
Faegri K, Van der Pilj L. The principles of pollination ecology. 3rd ed. Oxford, United Kingdom: Pergamon Press; 1979. 244 p.
Mejía D, Posada CE. Cocaine Production and Trafficking: What do we know?. Policy Research Working Paper No. 4618. Washington, DC, United States of America: The World Bank; 2008. 60 p. https://doi.org/10.1596/1813-9450-4618
Stanley EA. Application of palynology to establish the provenance and travel history of illicit drugs. Microscope. 1992; 40:149–52.
Cordeiro I, Secco R, Cardiel JM, Steinmann V, Caruzo MBR, Riina RG, Lima LR, Maya-L., CA, Berry P, Carneiro-Torres DS, Pscheidt AC. Euphorbiaceae. In: Forzza RC (Editor). Catálogo de plantas e fungos do Brasil. Rio de Janeiro, Brazil: Instituto de Pesquisas Jardim Botânico do Rio de Janeiro; 2010. p. 963-989.
Bovarnick A, Alpizar F, Schnell C (Editors). The Importance of Biodiversity and Ecosystems in Economic Growth and Equity in Latin America and the Caribbean: An economic valuation of ecosystems, United Nations Development Programme; 2010. 327 p.
Abreu, A. Cocaína: A Rota Caipira. O Narcotráfico no Principal Corredor de Drogas do Brasil. Rio de Janeiro, Brasil: Editora Record; 2017. 826 p.
Stockmarr J. Tablets with spores used in absolute pollen analysis. Pollen Spores. 1971; 13:615-21.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Brazilian Journal of Forensic Sciences, Medical Law and Bioethics
This work is licensed under a Creative Commons Attribution 4.0 International License.